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lambda embl3/bamhi vector kit  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda embl3/bamhi vector kit
    Lambda Embl3/Bamhi Vector Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+embl3+vector/10__4238_slash_2013__february__8__7-40-18-22
    Average 90 stars, based on 1 article reviews
    lambda embl3/bamhi vector kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family.
    Article Snippet: .. The following strains were used: H. mediterranei (ATCC 33500) [3], E. coli strain XL1-Blue MRA (P2) as the host for lambda EMBL3 vector (Stratagene), E. coli NovaBlue (Novagen) as host for plasmids pCR02.1 and pET3a, and E. coli BL21(DE3) (Novagen) as the expression host. .. Vector pCR02.1 was used for cloning the PCR product using the TA Cloning0 kit (Invitrogen).

    Article Title: Genomic organizations of two small subunit ADP-glucose pyrophosphorylase genes from sweetpotato.
    Article Snippet: The genomic features of the small subunit ADP-glucose pyrophosphorylase (AGPase) isoforms are different in barley and maize.. The two isoforms found in barley originated from one single gene through alternative splicing, while two independent genes encode the two isoforms in maize.. To ascertain the genomic organizations of two small subunit AGPase isoforms in sweetpotato (ibAGP1 and ibAGP2), we isolated genomic DNAs containing the entire coding regions of two genes.

    Article Title: DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.
    Article Snippet: .. DNA fragments of about 20 kb were isolated by ultracentrifugation at 26 000 r.p.m. for 24 h at 18 uC in a sucrose gradient (10–40 %), purified by precipitation with ethanol and ligated to BamHI-digested Lambda EMBL3 vector (Stratagene). .. Recombinant lambda phages were packaged by using the Lambda EMBL3/BamHI Gigapack III Gold Cloning kit (Stratagene).

    Article Title: Histone gene complement, variant expression, and mRNA processing in a urochordate Oikopleura dioica that undergoes extensive polyploidization.
    Article Snippet: .. This fragment was random prime labeled with 32P-a-dCTP and used to screen a genomic library that had been prepared by partial digestion of sperm DNA with Sau 3A and cloned into the lambda EMBL3 vector (Stratagene). ..

    Article Title: Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei.
    Article Snippet: Glutamate synthase (GOGAT) is one of the two important enzymes involved in the ammonium assimilation pathway glutamine synthetase (GS)/GOGAT, which enables Hfx. mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source.. The gene coding for this enzyme, gltS, has been sequenced, analysed and compared with other GOGATs from different organisms from the three domains of life.. According to its amino acid sequence, Hfx. mediterranei GOGAT displays high homology with those from other archaeal halophilic organisms and with the bacterial alpha-like subunit.

    Expressing:

    Article Title: Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family.
    Article Snippet: .. The following strains were used: H. mediterranei (ATCC 33500) [3], E. coli strain XL1-Blue MRA (P2) as the host for lambda EMBL3 vector (Stratagene), E. coli NovaBlue (Novagen) as host for plasmids pCR02.1 and pET3a, and E. coli BL21(DE3) (Novagen) as the expression host. .. Vector pCR02.1 was used for cloning the PCR product using the TA Cloning0 kit (Invitrogen).

    Construct:

    Article Title: Genomic organizations of two small subunit ADP-glucose pyrophosphorylase genes from sweetpotato.
    Article Snippet: The genomic features of the small subunit ADP-glucose pyrophosphorylase (AGPase) isoforms are different in barley and maize.. The two isoforms found in barley originated from one single gene through alternative splicing, while two independent genes encode the two isoforms in maize.. To ascertain the genomic organizations of two small subunit AGPase isoforms in sweetpotato (ibAGP1 and ibAGP2), we isolated genomic DNAs containing the entire coding regions of two genes.

    Article Title: Identification of a genomic clone to ACC oxidase from papaya (Carica papaya L.) and expression studies.
    Article Snippet: Instituto de Investigaciones Quı́mico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3 Ciudad Universitaria, Morelia, Mich. CP 58060, Mexico; Departamento de Quı́mica, Centro Básico, Universidad Autónoma de Aguascalientes, Avenida Universidad 940, C.P.. 20 100, Aguascalientes, Ags.. Mexico; and CINVESTAV-Irapuato, Km 9.6 carretera Irapuato-León, Apartado Postal 629, Irapuato GTO, Mexico

    Isolation:

    Article Title: DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.
    Article Snippet: .. DNA fragments of about 20 kb were isolated by ultracentrifugation at 26 000 r.p.m. for 24 h at 18 uC in a sucrose gradient (10–40 %), purified by precipitation with ethanol and ligated to BamHI-digested Lambda EMBL3 vector (Stratagene). .. Recombinant lambda phages were packaged by using the Lambda EMBL3/BamHI Gigapack III Gold Cloning kit (Stratagene).

    Article Title: Identification of a genomic clone to ACC oxidase from papaya (Carica papaya L.) and expression studies.
    Article Snippet: Instituto de Investigaciones Quı́mico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3 Ciudad Universitaria, Morelia, Mich. CP 58060, Mexico; Departamento de Quı́mica, Centro Básico, Universidad Autónoma de Aguascalientes, Avenida Universidad 940, C.P.. 20 100, Aguascalientes, Ags.. Mexico; and CINVESTAV-Irapuato, Km 9.6 carretera Irapuato-León, Apartado Postal 629, Irapuato GTO, Mexico

    Purification:

    Article Title: DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.
    Article Snippet: .. DNA fragments of about 20 kb were isolated by ultracentrifugation at 26 000 r.p.m. for 24 h at 18 uC in a sucrose gradient (10–40 %), purified by precipitation with ethanol and ligated to BamHI-digested Lambda EMBL3 vector (Stratagene). .. Recombinant lambda phages were packaged by using the Lambda EMBL3/BamHI Gigapack III Gold Cloning kit (Stratagene).

    Labeling:

    Article Title: Histone gene complement, variant expression, and mRNA processing in a urochordate Oikopleura dioica that undergoes extensive polyploidization.
    Article Snippet: .. This fragment was random prime labeled with 32P-a-dCTP and used to screen a genomic library that had been prepared by partial digestion of sperm DNA with Sau 3A and cloned into the lambda EMBL3 vector (Stratagene). ..

    Clone Assay:

    Article Title: Histone gene complement, variant expression, and mRNA processing in a urochordate Oikopleura dioica that undergoes extensive polyploidization.
    Article Snippet: .. This fragment was random prime labeled with 32P-a-dCTP and used to screen a genomic library that had been prepared by partial digestion of sperm DNA with Sau 3A and cloned into the lambda EMBL3 vector (Stratagene). ..

    Cloning:

    Article Title: Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei.
    Article Snippet: Glutamate synthase (GOGAT) is one of the two important enzymes involved in the ammonium assimilation pathway glutamine synthetase (GS)/GOGAT, which enables Hfx. mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source.. The gene coding for this enzyme, gltS, has been sequenced, analysed and compared with other GOGATs from different organisms from the three domains of life.. According to its amino acid sequence, Hfx. mediterranei GOGAT displays high homology with those from other archaeal halophilic organisms and with the bacterial alpha-like subunit.

    Polymerase Chain Reaction:

    Article Title: Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei.
    Article Snippet: Glutamate synthase (GOGAT) is one of the two important enzymes involved in the ammonium assimilation pathway glutamine synthetase (GS)/GOGAT, which enables Hfx. mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source.. The gene coding for this enzyme, gltS, has been sequenced, analysed and compared with other GOGATs from different organisms from the three domains of life.. According to its amino acid sequence, Hfx. mediterranei GOGAT displays high homology with those from other archaeal halophilic organisms and with the bacterial alpha-like subunit.

    In Vitro:

    Article Title: Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei.
    Article Snippet: Glutamate synthase (GOGAT) is one of the two important enzymes involved in the ammonium assimilation pathway glutamine synthetase (GS)/GOGAT, which enables Hfx. mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source.. The gene coding for this enzyme, gltS, has been sequenced, analysed and compared with other GOGATs from different organisms from the three domains of life.. According to its amino acid sequence, Hfx. mediterranei GOGAT displays high homology with those from other archaeal halophilic organisms and with the bacterial alpha-like subunit.



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